Journal: Journal of Lipid Research

Article Title: LYPLAL1 rare loss-of-function variants in humans and deletion in human hepatoma cells protect against MASLD

doi: 10.1016/j.jlr.2026.101013

Figure Lengend Snippet: LYPLAL1 knockout alters the expression of metabolic regulators and mitochondrial β-oxidation processes. A: mRNA levels of transcription factors MLXIPL and PPARα without oleic acid (OA) and with 200 μM OA. B and C: mRNA levels of (B) de novo lipogenesis genes and (C) β-oxidation genes with and without 200 μM OA treatment. D: Representative histograms and relative mean fluorescence intensity (MFI) showing protein levels of major β-oxidation enzymes ACADVL, ACADM, and HADHA without oleic acid and with 200 μM OA. E: Seahorse XF assay for mitochondrial oxygen consumption rate (OCR) in vehicle or etomoxir treated HuH-7 and LYPLAL1 KO cells without oleic acid and with 200 μM OA. Port A: Etomoxir (4 μM), port B: Oligomycin (1.5 μM), port C: FCCP (0.5 μM), and port D: Rotenone/antimycin A (0.5 μM). F: Maximal oxygen consumption rate (OCR) with vehicle or etomoxir treated HuH-7 and LYPLAL1 KO cells without oleic acid and with 200 μM OA. LYP KO = LYPLAL1 KO, Eto = Etomoxir. G: Vehicle treated (<1% DMSO) and etomoxir treated (100 μM) HuH-7 and LYPLAL1 KO cells with 200 μM OA. Cells were treated with etomoxir and oleic acid for 24 h. Data represented as mean ± S.E.M. ns: P > 0.05, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

Article Snippet: Probes used: GAPDH (Hs02786624_g1), ELF1 (Hs01111177_m1), MLXIPL (Hs00975714_m1), PPARα (Hs00947536_m1), ACACA (Hs01046047_m1), ACLY (Hs00982738_m1), FASN (Hs01005622_m1), SCD (Hs01682761_m1), CPT1A (Hs00912671_m1), ACADL (Hs00155630_m1), ACADM (Hs00936584_m1), ACADVL (Hs00825606_g1), EHHADH (Hs00157347_m1), HADHA (Hs00426191_m1), CD36 (Hs00354519_m1), and FABP1 (Hs00155026_m1).

Techniques: Knock-Out, Expressing, Fluorescence, XF Assay

Journal: The Journal of Biological Chemistry

Article Title: GALNT7-induced O-glycosylation of NUP50 activates fatty acid β-oxidation to promote lung adenocarcinoma metastasis

doi: 10.1016/j.jbc.2026.113179

Figure Lengend Snippet: O-Glycosylation of NUP50 plays a crucial role in LUAD cell metastasis. A , Pathway enrichment analysis of NUP50 using GSEA. B and C , Neutral lipid droplet content measured by flow cytometry in NUP50-KO Calu-3 cells with wild-type or mutant NUP50 treated with DMSO or ETX. D and E , NAD+/NADH and ATP levels were measured using assay kits to assess metabolic activity. F , expression of ACADS, MCAD, and LCAD analyzed by WB. G–I proliferation (CCK-8 assay), migration, and invasion (Transwell assays) were evaluated in NUP50-KO Calu-3 cells (H: scale bar = 200 μm). J , EMT and metastasis-related proteins detected by WB. ∗ indicates p < 0.05.

Article Snippet: Antibodies used included GALNT7 (ABclonal, A20259), NUP50 (ABclonal, A9441, China), E-cadherin (ABclonal, A20798), Vimentin (Proteintech, 60330-1-Ig), MMP-2 (ABclonal, A19080), MMP-9 (ABclonal, A25299), ACADS (ABclonal, A20926), MCAD (ABclonal, A4567), LCAD (ABclonal, A4884), Vicia Villosa Lectin (VVA) (Vector Laboratories, CAT# B-1235–2), and GAPDH (HUABIO, ET1601–4).

Techniques: Glycoproteomics, Flow Cytometry, Mutagenesis, Activity Assay, Expressing, CCK-8 Assay, Migration

Journal: The Journal of Biological Chemistry

Article Title: GALNT7-induced O-glycosylation of NUP50 activates fatty acid β-oxidation to promote lung adenocarcinoma metastasis

doi: 10.1016/j.jbc.2026.113179

Figure Lengend Snippet: GALNT7 knockdown rescues LUAD cell metastasis mediated by NUP50 glycosylation. A , VVA precipitation assays evaluating the impact of GALNT7 on NUP50 glycosylation in NUP50-KO Calu-3 cells: sh-NC+NUP50-MUT, sh-NC+NUP50-WT, or sh-GALNT7+NUP50-WT. B and C , neutral lipid droplet content quantified by flow cytometry. D and E , NADH ( D ) and ATP ( E ) levels in LUAD cells measured using NAD+/NADH and ATP content assay kits. F , ACADS, MCAD, and LCAD expressions analyzed by WB. G–I , proliferation (CCK-8), migration, and invasion (Transwell assays) assessed in NUP50-KO Calu-3 cells. J , EMT and metastasis-related proteins detected by WB. ∗ indicates p < 0.05.

Article Snippet: Antibodies used included GALNT7 (ABclonal, A20259), NUP50 (ABclonal, A9441, China), E-cadherin (ABclonal, A20798), Vimentin (Proteintech, 60330-1-Ig), MMP-2 (ABclonal, A19080), MMP-9 (ABclonal, A25299), ACADS (ABclonal, A20926), MCAD (ABclonal, A4567), LCAD (ABclonal, A4884), Vicia Villosa Lectin (VVA) (Vector Laboratories, CAT# B-1235–2), and GAPDH (HUABIO, ET1601–4).

Techniques: Knockdown, Glycoproteomics, Flow Cytometry, CCK-8 Assay, Migration